Gene therapy of virus replication with RNAi
نویسندگان
چکیده
منابع مشابه
Gene Therapy Using RNAi
In the chapter, we hereby provide a brief review about RNA interference (RNAi) and elucidate its therapeutic applications. The enzymatic synthesis of double-stranded RNA in vitro will be discussed. We also describe how to design and select short interfering RNA (siRNA) sequences. In addition, we also introduce a DNA-directed RNAi (ddRNAi) system, which is designed for the generation of small ha...
متن کاملRNAi and gene therapy: a mutual attraction.
The phylogenetically conserved cellular phenomenon of RNA interference (RNAi)-the sequence-specific post-transcriptional silencing of gene expression mediated by small double-stranded RNAs-holds substantial promise for basic research and for drug development. Particularly attractive from a medical standpoint is the juxtaposition of new RNAi methodology with established gene transfer strategies,...
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RNA interference (RNAi) is an important antiviral defense response in plants and invertebrates; however, evidences for its contribution to mammalian antiviral defense are few. In the present study, we demonstrate the anti-dengue virus role of RNAi in mammalian cells. Dengue virus infection of Huh 7 cells decreased the mRNA levels of host RNAi factors, namely, Dicer, Drosha, Ago1, and Ago2, and ...
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Gene delivery to the nervous system represents perhaps the ultimate challenge of gene therapy in view of the complexity of this system, the wide variety of intractable neurological diseases and the need to deliver the gene to non-dividing cells. Although a variety of systems for such gene delivery are under development, herpes simplex virus has unique advantages in terms of its large genome siz...
متن کاملVirus meets RNAi
Introduction Small rnas are important regulators of gene expression that operate through a range of rna-silencing mechanisms. of these, rna interference (rnai)—gene silencing through small-interfering rnas (sirnas) derived from exogenous double-stranded rna (dsrna)—and the microrna (mirna) pathway—gene silencing guided by small rnas encoded in the genome—are the best known. the discovery that d...
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ژورنال
عنوان ژورنال: Uirusu
سال: 2005
ISSN: 0042-6857,1884-3433
DOI: 10.2222/jsv.55.1